Pgem t easy manual promega fitchburg

 

 

PGEM T EASY MANUAL PROMEGA FITCHBURG >> DOWNLOAD LINK

 

PGEM T EASY MANUAL PROMEGA FITCHBURG >> READ ONLINE

 

 

 

 

 

 

 

 

 

 

Pgem T Easy Manual Promega Cell. Download Pgem T Easy Manual Promega Cell free and unlimited. Instruction manual_BOILER.pdf. The pGEM-T Easy Vector Systems are convenient systems for the cloning of PCR products. Thus several options for removal of the desired insert DNA with a single restriction digestion are provided. The pGEM-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM-T Kloning gen fim-c salmonella typhimurium dengan vektor pgem-t easy untuk pengembangan vaksin rekombinan penyakit typhus pada manusia. Reaksi ligasi dilakukan sesuai prosedur kit vektor pGEM-T easy. 2000. Instructional manual, Promega. Promega. Alt Name: pGEM®-T Easy, pGEM-T Easy. Analyze The only difference between pGEM-T and pGEM-T Easy is in the multiple cloning site (MCS). The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I pGEM®-T Easy. Antibiotic Resistance. Ampicillin. KEYWORDS pGEM-T Easy SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3015) AUTHORS Promega TITLE Direct Submission COMMENT Linearize at EcoRV to create a TA cloning vector. Cloning - Promega pGEM-T Easy kit Ligation 1. Add to the reaction mixture: a. 3 µl Fresh PCR product b. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase 2. Incubate at room temperature for 1 hour or overnight at 4°C 3. Store on ice until. Visit promega.com/customize. © 2018 Promega Corporation. • Easy to Prepare: Supplied as a dried substrate for easy preparation in methanol or ethanol. • Quality Tested: X-Gal is tested for use with the pGEM®-Z Vectors in a chromogenicity assay. Promega's pGEM-T vector systems are very dependable and versatile tools to routinely clone fragments of DNA into reliable plasmids. I primarily use the pGEM-T and pGEM-T Easy vectors for cloning PCR products. These can be further analyzed by direct sequencing, or used for more indirect • The Promega PCR Performance Guarantee. Protocol Technical Manual Quick Protocol Card. Part# TM318 FB103. Storage Conditions: Store all components at Description: The pGEM®-T Easy Vector Systems are convenient systems for the cloning of PCR products. They offer all of the advantages of E-mail Promega Technical Services if you have questions on use of this system: [email protected]m Promega Corporation · 2800 Woods Hollow Road · Madison, WI 3 3. Protocol for Ligations Using the pGEM ® -T and pGEM ® -T Easy Vectors and the 2X Rapid Ligation Bu ff er ..4 3.A. 2. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual #TM042. All Rights Reserved. pGEM and Wizard are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office. 2. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual #TM042. All Rights Reserved. pGEM and Wizard are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office.

Robot 2012 training manual metric measurements, How a front wheel drive manual transmission works, Delonghi gm6000 manual, Xc2288h user manual, Ontbinding bvba procedure manual template.